Part:BBa_K1758202:Design

His-tagged lac repressor fused to superfolder GFP under the control of the T7 promoter

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 1146
  

Design Notes

This is a composite part which enables a high protein expression and an easy purification of lacI fused to superfolding GFP (sfGFP). The green fluorescence of the culture is a sign for protein expression.

Source

We clone sfGFP (Bba_I746916) via Gibson Assembly in the device K1758201.